Atcc hl-60

The protocols I have read seem straightforward on differentiating HL-60 cells, however I am not having luck using 1.3% DMSO and/or 1uM ATRA (all trans retinoic acid). I culture in 1640 RPMI

Leukemia. 27.9. 468. ATCC website. No PMID.

31.03.2021 Atcc hl-60

Peripheral blood leukocytes were obtained by leukopheresis from a 36-year-old Caucasian female with acute promyelocytic leukemia. Jan 17, 1989 · Although intact ATCC HL-60 cells specifically and saturably take up 1,25-dihydroxy[3H]vitamin D-3, whole cell extracts have little or no specific binding of 1,25-dihydroxyvitamin D-3. The absence of specific binding can now be explained by the action of a serine proteinase in these cells. I cryopreserved HL-60 cells in FBS/ 10% DMSO but unfortunately cannot recover these cells properly. The cells have a good appearance at day 1 and 2. However, significantly lower cells counts are Aliases: HL-60.

HL-60/S4 (ATCC® CRL-3306™). MoreLess. Pricing.

96100920 . Human promyelocytic leukaemic cells established from HL60 cells by sub-cloning in methylcellulose.

Article Snippet: Cell lines The mycoplasma-free AML U937 (ATCC CRL-1593.2; French-American-British/FAB phenotype M5, monoblast), NB4 (M3, promyelocyte) [ ], HL-60 (ATCC 240-CCL; M2, myeloblast) and THP-1 (ATCC TIB-202; M5, monoblast) cells were cultured in complete RPMI 1640 medium supplemented with 5% FCS in a 5% CO2 humidified atmosphere at

Flow cytometry, Western blot and ELISA were used to measure cell cycle, cyclin D1, mPGES-1, PGE(2), 20/03/2018 Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH Human HL-60 cells (ATCC-No: CCL-240) were isolated from a patient with AML (acute myeloid leukemia) and express FLT3 wild type. A Hematoxylen-Eosin stained paraffin section of a subcutaneous HL-60 xenograft is shown on the right. As routine quality controls, the cells are regularly checked for Mycoplasma contamination and authenticity (via STR DNA Typing). Expression data using western … Article Snippet: Cell lines The mycoplasma-free AML U937 (ATCC CRL-1593.2; French-American-British/FAB phenotype M5, monoblast), NB4 (M3, promyelocyte) [ ], HL-60 (ATCC 240-CCL; M2, myeloblast) and THP-1 (ATCC TIB-202; M5, monoblast) cells were cultured in complete RPMI 1640 medium supplemented with 5% FCS in a 5% CO2 humidified atmosphere at Human Aml Cell Lines Hl60, supplied by ATCC, used in various techniques.

96100920 .

As routine quality controls, the cells are regularly checked for Mar 24, 2005 · HL‐60 cell culture, differentiation and labelling. The HL‐60 cell line was obtained from ATCC and maintained in Iscove's modified Dulbecco's medium (Invitrogen, Grand Island, NY) supplemented with 10% fetal bovine serum, 50 U/ml penicillin, and 50 µg/ml streptomycin at 37° with 5% CO 2. Clone, HL-60, HL60 Human caucasian promyelocytic leukaemia The clone 15 HL-60 has been derived from HL-60 cells (ECACC catalogue no. 98070106) that had been cultured at an elevated pH of 7.6 - 7.8 for 2 months. Clone 15 HL-60 was picked from soft agar being selected for the ability to differentiate to eosinophils after treatment with butyric acid.

(A) The presence of acidic vesicles was revealed using Cyto-ID. Data were represented as the % of Cyto-ID-positive cells, in Annexin-V-/7AAD- cells, relative to control cells. Eos-HL-60 . 96100920 . Human promyelocytic leukaemic cells established from HL60 cells by sub-cloning in methylcellulose. Although the cells are capable of reverting to the parental phenotype, most passages maintain a high degree of eosinophil differentiation.Eos-HL-60 and HL-60 have been shown to be identical genetically by STR . July 2018 HL-60 (ATCC® CCL-240™).

7500 33 HL -60. 40,000. Leukemia. 28.6. 1250. 2.

Unopsonized and opsonized A. actinomycetemcomitans and W. recta had equally potent lethal effects on induced HL-60 cells. … I got a HL-60 cells from ATCC and cultured them in IMEM high glucose+20% FBS (non heat inactivated as recommend by atcc technical support)and cryopreserved the cells at a density if 4*105 cells/ml. Eos-HL-60 . 96100920 . Human promyelocytic leukaemic cells established from HL60 cells by sub-cloning in methylcellulose.

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DNA purified from ATCC CCL-240, HL-60, acute promyelocytic leukemia cell line . MoreLess. Pricing. For-Profit: $163.00; Non

Clom 15 ATCC FAQ #11058. No PMID Leukemia, chronic myeloid. CVCL_8439.

Eos-HL-60 . 96100920 . Human promyelocytic leukaemic cells established from HL60 cells by sub-cloning in methylcellulose. Although the cells are capable of reverting to the parental phenotype, most passages maintain a high degree of eosinophil differentiation.Eos-HL-60 and HL-60 have been shown to be identical genetically by STR . July 2018

The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology.

Disease ATCC Gender Female HL60 sequenced and profiled in following datasets. Loss-of-function screens Combined RNAi (Broad, Novartis, Marcotte) Article Snippet: HL-60 culture under DMSO and ATRA exposure HL-60 cells (CCL-240, ATCC, Manassas, VA) were either cultured in RPMI 1640 containing with 10% heat-inactivated fetal bovine serum (FBS), 1% penicillin/streptomycin antibiotics or RPMI 1640 containing 2% FBS, 2% Nutridoma and 5 mL % penicillin/streptomycin antibiotics at 37 °C in 5% Eos-HL-60 . 96100920 . Human promyelocytic leukaemic cells established from HL60 cells by sub-cloning in methylcellulose. Although the cells are capable of reverting to the parental phenotype, most passages maintain a high degree of eosinophil differentiation.Eos-HL-60 and HL-60 have been shown to be identical genetically by STR .